pax2 antibody Search Results


goat anti pax2  (R&D Systems)
99
R&D Systems goat anti pax2
Goat Anti Pax2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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99/100 stars
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goat anti pax2 antibody  (Bio-Techne corporation)
94
Bio-Techne corporation goat anti pax2 antibody
( A ) GFP (green) and <t>PAX2</t> (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.
Goat Anti Pax2 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti pax2 antibody/product/Bio-Techne corporation
Average 94 stars, based on 1 article reviews
goat anti pax2 antibody - by Bioz Stars, 2026-03
94/100 stars
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pax 2  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology pax 2
( A ) GFP (green) and <t>PAX2</t> (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.
Pax 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pax 2/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
pax 2 - by Bioz Stars, 2026-03
93/100 stars
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polyclonal goat anti pax2  (R&D Systems)
93
R&D Systems polyclonal goat anti pax2
( A ) GFP (green) and <t>PAX2</t> (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.
Polyclonal Goat Anti Pax2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal goat anti pax2/product/R&D Systems
Average 93 stars, based on 1 article reviews
polyclonal goat anti pax2 - by Bioz Stars, 2026-03
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rabbit anti pax2  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc rabbit anti pax2
( A ) GFP (green) and <t>PAX2</t> (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.
Rabbit Anti Pax2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti pax2/product/Cell Signaling Technology Inc
Average 94 stars, based on 1 article reviews
rabbit anti pax2 - by Bioz Stars, 2026-03
94/100 stars
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anti pax2 antibody  (Novus Biologicals)
91
Novus Biologicals anti pax2 antibody
( A ) GFP (green) and <t>PAX2</t> (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.
Anti Pax2 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti pax2 antibody/product/Novus Biologicals
Average 91 stars, based on 1 article reviews
anti pax2 antibody - by Bioz Stars, 2026-03
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pbsg pax2 santa cruz sc  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology pbsg pax2 santa cruz sc
( A ) GFP (green) and <t>PAX2</t> (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.
Pbsg Pax2 Santa Cruz Sc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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anti pig pax2  (Atlas Antibodies)
86
Atlas Antibodies anti pig pax2
( A ) GFP (green) and <t>PAX2</t> (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.
Anti Pig Pax2, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
anti pig pax2 - by Bioz Stars, 2026-03
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anti pax2  (Boster Bio)
92
Boster Bio anti pax2
( A ) GFP (green) and <t>PAX2</t> (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.
Anti Pax2, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti pax2/product/Boster Bio
Average 92 stars, based on 1 article reviews
anti pax2 - by Bioz Stars, 2026-03
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anti pax2  (OriGene)
90
OriGene anti pax2
( A ) GFP (green) and <t>PAX2</t> (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.
Anti Pax2, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
anti pax2 - by Bioz Stars, 2026-03
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rabbit  (Boster Bio)
92
Boster Bio rabbit
( A ) GFP (green) and <t>PAX2</t> (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.
Rabbit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit/product/Boster Bio
Average 92 stars, based on 1 article reviews
rabbit - by Bioz Stars, 2026-03
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pax-2 antibody  (AbCys s a)
90
AbCys s a pax-2 antibody
( A ) GFP (green) and <t>PAX2</t> (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.
Pax 2 Antibody, supplied by AbCys s a, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) GFP (green) and PAX2 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.

Journal: Science Advances

Article Title: Switch of serotonergic descending inhibition into facilitation by a spinal chloride imbalance in neuropathic pain

doi: 10.1126/sciadv.abo0689

Figure Lengend Snippet: ( A ) GFP (green) and PAX2 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.

Article Snippet: For molecular identifications of the spinal network, spinal cord sections were incubated free-floating in 0.1 M PBS containing Triton X-100 (0.3%), bovine serum albumin (1%; Sigma-Aldrich), guinea pig anti-TLX3 antibody (1:1000; a gift from T. Müller), goat anti-Pax2 antibody (1:300; Bio-techne), rabbit anti-Myc antibody (1:500; Euromedex), rabbit anti-TPH2 antibody (1:1000; Bio-techne), and rabbit anti-GAD65/67 (G5163, Sigma-Aldrich) in combination with chicken anti-GFP antibody (1:1000; Averlabs) overnight at 4°C.

Techniques: Immunolabeling, MANN-WHITNEY, Immunostaining, Expressing